FastQCFastQC Report
Sun 7 Aug 2016
GBM44.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM44.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences699621
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1600.052.6582871
AGAGCGT851.4733814E-1049.61758
TCGGAAG1650.045.385723
TCGGGAA952.194247E-839.4139183
ATCGGGA1359.094947E-1238.1366122
GTACCTT500.001736851937.4432223
GACCGGA500.001736851937.4432222
TCGAATC500.001736851937.4432222
TTATCCG2150.037.005194
AAGAGCG1650.036.9207657
CGTATCG2450.036.2969973
TATCCGG2200.036.158995
TCCGGTA2300.034.636377
CTTCGTT550.002771530834.041721
CCGAAAG1107.971539E-834.041721
CGGAAGA2100.033.4290544
GCCGTAT2550.033.0404931
GTATCGT2700.032.933814
ATCCGGT2450.032.5157746
CCGGTAA2450.032.5157748
Produced by FastQC (version 0.11.2)