FastQCFastQC Report
Sun 7 Aug 2016
GBM59.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM59.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99372724
Sequences flagged as poor quality0
Sequence length101
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG98050.018.2105523
GTATCGA134850.014.9840374
CCCGTAT183600.014.3011911
CCGTATC163100.013.8299222
TACCGTA112700.012.0729179
TAAGGCG202200.011.1778788
CTACCGT132550.010.7765048
CTTGGAT714050.010.1043391
CTCGGAT223350.09.8068051
CAATCGG116600.09.3818634
CGGGGAT220400.09.3207951
ACTACCG161200.09.3098697
ATCGAAG281500.09.281576
CTCGGTT157850.08.9634731
CTCCGAT237100.08.6834161
CTGGAAT1010700.08.6821051
AATCGGG150000.08.6790715
GTCGGTT224000.08.664871
CTCAGAT715050.08.5744571
CGGGGTT269350.08.519221
Produced by FastQC (version 0.11.2)