FastQCFastQC Report
Sun 7 Aug 2016
GBM59.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM59.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99372724
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG100250.018.966673
CCCGTAT185450.015.1223461
GTATCGA134600.014.9929444
CCGTATC174550.013.6414032
TACCGTA108100.011.9088779
TAAGGCG207750.011.1625628
CAATCGG129150.010.0698524
CTACCGT131450.09.9299448
CTTGGAT732150.09.8977951
ATCGAAG276500.09.4719786
CTCCGAT244150.09.3730171
CTCGGAT235500.09.3552751
AATCGGG155800.09.2399765
CTGGAAT1003850.09.0291841
CTCAGAT721750.08.9524451
ACTACCG160850.08.6154197
CTCGAAT223350.08.5985211
CTCGGTT160400.08.5042331
CGGGGAT229550.08.4249231
CGACCGT55650.08.3855991
Produced by FastQC (version 0.11.2)