FastQCFastQC Report
Sun 7 Aug 2016
GBM6.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM6.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83439125
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG74150.015.0369423
CCCGTAT120950.011.9839411
TGCGCGA85100.011.8301689
GTATCGA94900.010.4599154
CTCGGAT216000.010.3271241
CCGTATC129200.010.30562
CAATCGG102800.09.7933794
CCGGTAT141600.09.6712931
CTCCGAT185400.09.6709711
CTCGGTT142350.09.5872781
TCGATCG65250.09.5170018
CTCAGAT615950.09.5121511
CTTGGAT628500.09.3596491
CTCGCAT231150.09.3448771
ACGTGCG136550.09.0954826
CTGGAAT905250.09.0195681
CGATCGG72350.08.9081759
GACGCTA93550.08.9010524
CTCGAAT192800.08.81160451
CTCGTTT190000.08.7185421
Produced by FastQC (version 0.11.2)