FastQCFastQC Report
Sun 7 Aug 2016
GBM61.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM61.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1326109
Sequences flagged as poor quality0
Sequence length101
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTA2100.047.0008132
TCAGACG2500.043.0698362
ATCCGGT3300.042.145366
TTATCCG3500.041.018894
AGGCGTA3050.039.7160388
CCGAAAG2500.039.6230851
CCGGTAA3650.039.3331838
TATCCGG3600.038.6332475
GCCGTAT2750.037.5229643
TGTTACG3600.037.3870123
TCCGGTA3850.037.28997
CTGCCGT2900.037.2630921
CGGTAAA3750.037.0893179
CAGGCGT3200.036.4523357
CGTCAGT500.002101201336.020991
TTACGAC3900.035.6614575
CCTCACC14200.035.5136531
TTTATCC4050.034.340663
GTTACGA4100.033.921874
AGACGTG3200.033.648314
Produced by FastQC (version 0.11.2)