FastQCFastQC Report
Sun 7 Aug 2016
GBM76.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM76.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58615805
Sequences flagged as poor quality0
Sequence length101
%GC49

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT70700.011.0952821
ATCGAAG167700.010.1340156
CGTATCG47650.09.7701793
CGCGTAA32800.09.676891
TCGATCG59200.09.5436138
CCGACAT151000.09.489031
CCGGTAT104050.08.62848951
TCGAAGG203850.08.4698787
CCGTATC81850.08.3937082
CTCAGAT410850.08.144881
TTCGCGC47500.08.0881627
CTCGGTT104850.07.95721441
CTCGGAT147750.07.94847731
CTCGTAT69400.07.90564971
CATCGAA155100.07.8974025
CGATCGG72800.07.8228069
CTGGAAT604850.07.8189471
GTATCGA57050.07.7642084
TCGCATT123700.07.74631452
CTCGCAT160600.07.7360041
Produced by FastQC (version 0.11.2)