FastQCFastQC Report
Sun 7 Aug 2016
GBM80.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM80.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103535978
Sequences flagged as poor quality0
Sequence length101
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG125500.012.7951163
CCCGTAT145800.012.3831211
CCGGTAT180000.012.0716751
GTATCGA114600.011.9596084
ATCGAAG313150.011.8879396
CGCGTAA72150.011.568741
CCGACAT254500.010.2668981
TGCGCGA119350.010.1950429
CCGTATC186900.010.1406162
TCGAAGG360350.09.9793397
CTCGGAT272850.09.609671
CGGTATT207350.09.5113482
ACGTGCG194300.08.5671086
CTCGGTT182200.08.4402611
TATCGAA180750.08.3334555
GCGTAAC107050.08.3241362
ACATCGA319700.08.2062434
CGCGTAT24400.07.99435041
CATCGAA295950.07.88661245
TTCGCGC83100.07.83827737
Produced by FastQC (version 0.11.2)