FastQCFastQC Report
Sun 7 Aug 2016
GBM80.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM80.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103535978
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG139500.012.2612083
CCCGTAT144700.012.2556671
CCGGTAT176950.012.0264141
GTATCGA114800.011.8880614
ATCGAAG304750.011.62361056
CGCGTAA71250.010.4599971
CCGACAT256300.010.0372411
CCGTATC202350.09.8726852
CGGTATT205550.09.7408282
TGCGCGA111350.09.4352459
TCGAAGG363700.09.3944927
CTCGGAT278100.09.3311511
CTCCGAT218300.08.9257421
CTCGCAT323950.08.7172811
CTCGGTT187800.08.5106521
TTCGCGC95500.08.5095617
TATCGAA179900.08.335085
CGCCAAT150600.08.31741
CTCGACT214700.08.3016961
CTGGAAT1065300.08.0705891
Produced by FastQC (version 0.11.2)