FastQCFastQC Report
Sun 7 Aug 2016
GBM81.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM81.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18552603
Sequences flagged as poor quality0
Sequence length101
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG19250.017.8609773
TACCGTA22800.016.8656889
GTATCGA25800.014.2031724
CCCGTAT36300.013.8722551
CCGTATC30300.013.7362312
CTACCGT30050.013.6998858
CGCGTAT4407.198669E-712.3725521
ACTACCG37850.010.7571357
GCGAACG7555.32782E-910.1864843
ATCGAAG52950.010.0817536
CTCGTTT35200.09.6660571
CTCCGAT41600.09.3785531
CGCGTAA8352.6411726E-89.23619651
GACGCTA25850.09.100454
TAAGGCG41450.08.8405758
CCGGTAA15150.08.6839261
CCGCAAT21500.08.6511951
CCCGGAT42400.08.4526311
ACCGTAT23250.08.36694110-11
CGACCTA10853.106834E-98.3391214
Produced by FastQC (version 0.11.2)