FastQCFastQC Report
Sun 7 Aug 2016
GBM81.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM81.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18552603
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG20150.018.0468773
CCCGTAT35400.014.0772461
GTATCGA27300.013.3202424
CCGTATC34350.013.0694452
GACGCTA23950.012.3716664
TACCGTA21950.012.0687879
GATCGGA19050.011.0764561
CTCCGAT40450.010.8769091
ACGCTAC28650.010.4993175
CTACCGT28800.09.6659688
CCCGGAT43950.09.6021151
CCCGGTT25550.09.4885711
CCGGTCG23400.09.20910553
CCGGTAA17400.09.0306021
TCCGATT34800.09.030312
ATCGAAG51650.08.953366
TAAGGCG42650.08.5273118
CTCGTTT36400.08.5103161
GTTCGAT28600.08.4766781
CCCGTCG20200.08.4455941
Produced by FastQC (version 0.11.2)