FastQCFastQC Report
Thu 12 Jan 2017
G143-M10-Index-12_CTTGTA_BC9WPLANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG143-M10-Index-12_CTTGTA_BC9WPLANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22023582
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC696910.3164380798727473TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC86500.039.04499444
TCGTATG86400.038.9123643
CTCGTAT84550.038.77683342
TCTCGTA80800.038.7556741
AATCTCG83250.037.69527439
ATCTCGT87400.035.879740
CGTCTGA107350.032.22630716
ACGTCTG110650.031.18538915
ACACGTC114000.030.34740813
GTAATCT104500.030.21992337
CACGTCT123400.028.03390314
ATCGGAA125650.027.369822
TGTAATC117900.026.76693536
GATCGGA129150.026.6184311
TAATCTC119700.026.49195138
TCGGAAG130550.026.3088443
CACACGT131950.026.28538512
GCACACG134250.025.83446911
CGGAAGA133600.025.7253674
CCTTGTA134250.025.68464533
Produced by FastQC (version 0.11.2)