FastQCFastQC Report
Thu 12 Jan 2017
G143-M10-Index-12_CTTGTA_BC9WPLANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG143-M10-Index-12_CTTGTA_BC9WPLANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22023582
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG546190.2480023458490994Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGC75100.037.01638842
CGTCGTG80700.036.6821812
GATCTCG82000.036.27948434
GCGTCGT81500.036.17831811
GTGGTCG78750.035.91389541
GTCGCCG78750.035.84415444
AGCGTCG82250.035.481710
TCGTGTA83000.035.22005514
TCTCGGT84100.035.1240336
ATCTCGG85050.034.6566435
CGTGTAG85600.034.23336815
GTCGTGT88000.034.22216813
TCGGTGG89300.033.57401738
CGGTGGT87100.033.39860539
GGTCGCC85750.033.0887343
GAGCGTC91250.031.9902069
AGAGCGT94600.031.0067148
CTCGGTG95250.030.9440437
AAGAGCG97050.030.0418157
ATCGGAA101500.029.8409942
Produced by FastQC (version 0.11.2)