FastQCFastQC Report
Thu 12 Jan 2017
G143-M11-Index-13_AGTCAA_BC9WPLANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG143-M11-Index-13_AGTCAA_BC9WPLANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21930173
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG374530.17078296646360244Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG55550.037.0619744
TGGTCGC56150.036.5356842
CGTCGTG59200.036.45211412
GCGTCGT59750.036.07040811
GTGGTCG59350.035.120841
AGCGTCG60100.034.99021510
GATCTCG61600.034.36507834
TCGTGTA62650.034.1949114
CGTGTAG64000.033.54732515
TCTCGGT64150.033.36105336
GTCGTGT65100.033.1911613
TCGGTGG65100.033.14753738
ATCTCGG64500.033.01246335
GGTCGCC63800.032.32977743
GAGCGTC66800.031.5868289
CGGTGGT68350.031.06803339
CTCGGTG71000.030.1737437
AGAGCGT71100.030.1192638
AAGAGCG74750.028.3832557
ATCGGAA79250.027.4010312
Produced by FastQC (version 0.11.2)