FastQCFastQC Report
Thu 12 Jan 2017
G143-M12-Index-14_AGTTCC_BC9WPLANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG143-M12-Index-14_AGTTCC_BC9WPLANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27190628
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG530150.1949752686844894Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG75250.036.01876444
TGGTCGC76200.035.6458742
GTGGTCG78950.035.1555241
GATCTCG80500.034.6885334
CGTCGTG83750.034.05465712
GCGTCGT83600.034.05366511
ATCTCGG84300.033.00853335
TCGTGTA86650.032.9991614
AGCGTCG85650.032.81075310
TCTCGGT86450.032.56622736
GTCGTGT91000.031.8113513
TCGGTGG89650.031.7497938
GGTCGCC85200.031.7271543
CGTGTAG91500.031.64040815
CGGTGGT88950.031.24495939
GAGCGTC95550.029.3515689
CTCGGTG96500.029.08408737
AGAGCGT103250.027.2790168
AAGAGCG107600.026.0131557
GTAGATC110900.025.85817531
Produced by FastQC (version 0.11.2)