FastQCFastQC Report
Wed 7 Dec 2016
G143-M7-M14-Index-8_ACTTGA_BC9WPLANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG143-M7-M14-Index-8_ACTTGA_BC9WPLANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42160107
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC2118070.5023872448900568Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC1555760.3690123461973187Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC1112130.26378728118503114TruSeq Adapter, Index 8 (100% over 50bp)
TGGAAGACTAGTGATTTTGTTGTTAGATCGGAAGAGCACACGTCTGAACT891640.21148902681864634Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACT820790.19468404100587317Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA763700.18114280402561597Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG724420.17182593962581735No Hit
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC649060.15395122218261922TruSeq Adapter, Index 5 (100% over 35bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT591900.1403933818289408No Hit
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACGTCTGAACTCC569990.13519652594809592Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCGGAAACAGGGACGAGCCCCTAGATCGGAAGAGCACACGTCTGAACTC556920.13209643893930345Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG501020.11883745930720716Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT471680.11187827393322318No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC454650.10783891037088686Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC436130.10344613214572725No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGAT206800.041.060896
GCTTATC531400.040.9026833
TCCGATG213650.039.8108527
GGTCCGA246750.039.7003365
AGCTTAT549750.039.5369762
CGATGGT214700.039.4668969
TAGCTTA552400.039.434091
CGTATGC132100.039.38775644
TATCGGG93750.038.823951
TGGTCCG255050.038.6556134
CTTATCA567400.038.451474
CCGATGG222200.038.430338
TCCGGAA267800.038.4208761
TGTTGAC454850.038.15413717
CGCGACC538600.038.014611
ACCCGTA87600.037.992682
GCACTAC243450.037.522886
GCGACCT543100.037.4700852
ACGTTCG41900.037.38586424
AACCGAC78800.037.22404510
Produced by FastQC (version 0.11.2)