FastQCFastQC Report
Thu 12 Jan 2017
G143-M7-M14-Index-8_ACTTGA_BC9WPLANXX_L007_001.R1_val_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG143-M7-M14-Index-8_ACTTGA_BC9WPLANXX_L007_001.R1_val_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32574859
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTCG11350.021.09550725
CGTTCGG13450.017.63941626
CGATAAC15700.017.48368640
TCGTACG5350.017.2978731
GCGATAA16150.016.86094339
CGTACGA6100.014.8055132
AGCGATA17900.014.721838
AAGCGAT21400.013.03252737
GTTCGGC20450.012.5683427
GGTCCGT19300.012.40657426
GCCGTCA26350.010.66922229
GTGCCGT26600.010.48835727
AATCTCG24550.010.21536821
AGTGCCG29350.09.87980826
CCGTCAG29550.09.66249430
TGCCGTC30250.09.4395528
TTTACCG15200.09.39121534
TGGTCCG26400.09.15267625
CGTCGAT6050.09.07583632
CGATCTA16350.09.0223138
Produced by FastQC (version 0.11.2)