FastQCFastQC Report
Thu 12 Jan 2017
G143-M7-M14-Index-8_ACTTGA_BC9WPLANXX_L007_001.R2_val_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG143-M7-M14-Index-8_ACTTGA_BC9WPLANXX_L007_001.R2_val_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32574859
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA11350.022.09202837
CCGAACG12600.020.2490836
GTTATCG16150.016.76741226
TATCGCT17050.015.8823328
TTATCGC16900.015.76285327
GTGTCGA19850.014.05602511
GATCGCA19150.013.90328834
ATCGCTT20050.013.72485329
ACGGCAC23650.012.65373339
GCCGAAC20550.012.63054735
CGAGATT21850.012.28914341
AGATCGC23350.012.15642133
ATCGCAG23050.011.64235435
CGACGCT14800.011.1385943
CCGTCAA18900.011.06044839
TGACGGC25350.011.01925837
ACGGACC22100.010.84901138
CATCCGA23250.010.7778829
ACGAGAT25450.010.72389140
CGGCACT27200.010.51944840
Produced by FastQC (version 0.11.2)