FastQCFastQC Report
Thu 12 Jan 2017
G143-M8-Index-9_GATCAG_BC9WPLANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG143-M8-Index-9_GATCAG_BC9WPLANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32029970
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC872020.2722512696702495TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG110950.039.60510343
CTCGTAT108000.039.52791242
CGTATGC112600.039.23921244
TCTCGTA104950.038.79359441
CGATCAG116400.038.09538333
ACGATCA117550.037.7423832
GTCACGA120600.036.7152529
CACGATC122550.036.2921131
GATCTCG112550.036.19507239
ATCTCGT113000.036.0891840
TCACGAT123550.035.99859230
AGTCACG130250.034.0978528
CGTCTGA131450.033.8586216
ACGTCTG134900.033.10725815
ACACGTC135200.032.92078413
CACGTCT150450.029.91915514
CACACGT159700.028.0358612
ATCGGAA158950.027.832082
GATCGGA162150.027.3439351
GCACACG167650.026.71918711
Produced by FastQC (version 0.11.2)