FastQCFastQC Report
Thu 12 Jan 2017
G143-M8-Index-9_GATCAG_BC9WPLANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG143-M8-Index-9_GATCAG_BC9WPLANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32029970
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG703500.21963804524325184Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG106850.037.06133712
TGGTCGC101750.036.88299642
GTCGCCG103350.036.43131344
GCGTCGT109800.036.2161611
GATCTCG107300.036.09303734
GTGGTCG107400.035.63765341
AGCGTCG110500.035.27902210
TCGTGTA112950.035.0526514
GTCGTGT115650.034.7821313
TCTCGGT115450.034.06418236
CGTGTAG116400.034.00093515
ATCTCGG114250.033.92381335
TCGGTGG118500.033.39394838
GGTCGCC113250.033.15947343
CGGTGGT118150.032.9282939
GAGCGTC118950.032.7279
AGAGCGT126750.030.912888
CTCGGTG127150.030.80922337
AAGAGCG131400.029.5680227
GTAGATC141800.027.73460831
Produced by FastQC (version 0.11.2)