FastQCFastQC Report
Thu 12 Jan 2017
G143-M9-Index-11_GGCTAC_BC9WPLANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG143-M9-Index-11_GGCTAC_BC9WPLANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21603624
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG434570.20115606529719274Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGC63600.036.8586642
CGTCGTG70900.035.67477812
GTCGCCG66600.035.64041544
GTGGTCG67700.035.37285641
GCGTCGT71500.035.18179311
GATCTCG69450.034.85262334
TCGTGTA71650.034.7203614
AGCGTCG70700.034.49892410
TCTCGGT72150.034.1116236
ATCTCGG71900.033.8965735
GTCGTGT75000.033.8505413
TCGGTGG75950.033.01672738
CGTGTAG76000.032.6801915
GGTCGCC73100.032.22127543
CGGTGGT77050.031.73077439
CTCGGTG79750.030.80648637
GAGCGTC80350.030.4195449
AGAGCGT82900.029.5467978
AAGAGCG87200.027.7360677
GTAGATC90600.027.10887731
Produced by FastQC (version 0.11.2)