FastQCFastQC Report
Thu 12 Jan 2017
G143_M10M11M12.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG143_M10M11M12.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71144383
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG103250.032.755843
CGTATGC103850.032.69329544
CCGTATC91750.032.60180738
CGTCTGA284300.031.94400616
TCCGTAT99250.031.11245337
CGTATCT98000.030.61212339
TATCTCG98550.030.28451741
ACGTCTG301500.030.10706115
ACACGTC303750.029.9357213
AGTTCCG115450.028.28846434
CACGTCT339200.026.89669414
TTCCGTA124100.026.45787836
GTTCCGT125500.026.16313435
ATCGGAA346250.025.9821832
CACACGT359950.025.47583412
GATCGGA354050.025.4106831
TCGGAAG358350.025.1479933
CGGAAGA363000.024.844424
GCACACG369250.024.7917511
CTCGTAT222150.023.64670244
Produced by FastQC (version 0.11.2)