FastQCFastQC Report
Thu 12 Jan 2017
G143_M10M11M12.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG143_M10M11M12.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71144383
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1450870.2039331762846267Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGC206600.036.61739342
GTCGCCG210750.036.1799244
CGTCGTG220900.036.06245812
GCGTCGT223750.035.59409711
GATCTCG222000.035.38482334
GTGGTCG217450.035.3553241
AGCGTCG226300.034.77337610
TCGTGTA230050.034.3157914
TCTCGGT234400.033.70511236
ATCTCGG234200.033.53875435
GTCGTGT242050.033.33854313
CGTGTAG241000.032.8438815
GGTCGCC233650.032.76653343
TCGGTGG245750.032.67920338
CGGTGGT245300.032.0161539
GAGCGTC252350.031.2528239
CTCGGTG262950.030.07151837
AGAGCGT265150.029.9120548
AAGAGCG273750.028.6745727
GTAGATC291300.027.35699831
Produced by FastQC (version 0.11.2)