FastQCFastQC Report
Thu 12 Jan 2017
G143_M7-M12.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG143_M7-M12.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157352836
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG409700.035.26687212
TGGTCGC391750.034.9758742
GCGTCGT416000.034.76038411
AGCGTCG419350.033.91406210
GTGGTCG416850.033.67598341
GTCGCCG409550.033.57460444
TCGTGTA427300.033.38276314
GATCTCG435650.032.7248434
GTCGTGT454750.032.1402613
TCTCGGT461350.031.42648536
ATCTCGG460800.031.17558735
TCGGTGG481350.030.4661438
GGTCGCC457050.030.08197243
CGGTGGT477400.030.05730239
CGTGTAG481000.029.78882415
GAGCGTC477950.029.6909459
AGAGCGT505700.028.2837778
CTCGGTG519700.027.79700537
AAGAGCG525250.027.013017
ATCGGAA566450.025.7808042
Produced by FastQC (version 0.11.2)