FastQCFastQC Report
Thu 12 Jan 2017
G143_M7M8M9.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG143_M7M8M9.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86208453
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG195350.037.0527643
CGTATGC199450.036.34601244
CTCGTAT193450.036.33822642
TCTCGTA190450.035.46936441
CGATCAG138050.031.96314833
ACGATCA141150.031.49566332
ATCTCGT219850.030.73660740
GTCACGA146550.030.35020429
AGTCACG242600.030.03345528
TCACGAT154100.028.89172730
CACGATC155450.028.55595831
ACGGCTA100050.028.07854332
GATCTCG148650.027.64206939
CGGCTAC103400.027.14665633
CGTCTGA285700.025.7157416
ACGTCTG304200.024.202715
ACACGTC306900.023.9620113
ATCGGAA316300.023.1140522
GATCGGA323650.022.6022431
CGGAAGA334650.021.8864064
Produced by FastQC (version 0.11.2)