FastQCFastQC Report
Thu 12 Jan 2017
G143_M7M8M9.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG143_M7M8M9.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86208453
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG184850.034.7254712
GCGTCGT188050.034.21906711
AGCGTCG190700.033.1057810
TGGTCGC185650.032.9009242
TCGTGTA195850.032.4357414
GTGGTCG197550.031.63045141
GTCGCCG197900.031.08585544
GTCGTGT210800.030.90827413
GATCTCG208500.030.41507134
TCTCGGT221450.029.26941736
ATCTCGG222850.028.78225735
GAGCGTC223000.028.3388029
TCGGTGG232200.028.1158838
CGGTGGT230950.027.7740339
GGTCGCC222950.027.58590543
CGTGTAG235950.027.05797415
AGAGCGT236050.026.7898838
AAGAGCG245400.025.4732137
CTCGGTG254200.025.36056737
ATCGGAA262600.024.7257882
Produced by FastQC (version 0.11.2)