FastQCFastQC Report
Wed 7 Dec 2016
G144-M10-Index-22_CGTACG_BC9WPLANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG144-M10-Index-22_CGTACG_BC9WPLANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43521583
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG15300.014.222651533
CGTACGT17200.012.26816534
CGTAATC16950.010.50309438
GTACGTA21900.010.337831535
ACCGTAC22750.09.95174332
CTCGTAT21900.09.5333644
ACGACGC21500.09.53222216
TCTCGTA23550.08.77218743
TACACGA27950.08.59465313
TCCGATC25300.08.34136126
CACGACG24600.08.33115115
CGACGCT23800.08.14807217
TACGTAA27900.07.956682736
CGGTTTT52850.07.91907021
ACGTAAT26050.07.84660237
CACCGTA31050.07.71628431
CCGATCT29850.07.585446427
CTACACG33700.06.997421312
TTCCGAT32800.06.96979925
ATCTCGT30700.06.65766642
Produced by FastQC (version 0.11.2)