FastQCFastQC Report
Wed 7 Dec 2016
G144-M7-Index-19_GTGAAA_BC9WPLANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG144-M7-Index-19_GTGAAA_BC9WPLANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40666652
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT19900.010.27125444
TCTCGTA21700.08.81166443
AACGATC22450.08.32192738
ACGATCT26050.07.17186339
TTACGCG8201.07320375E-106.986554616
ATCGACG5901.4910638E-66.70554941
TACGCGA8003.248715E-96.61011117
GATCTCG29150.06.559859341
TGAAACG33950.06.539420635
ATCTCGT29400.06.50391142
CGATCTC27850.06.471408440
ACGACGC18250.06.157592316
GAAACGA40900.05.85786736
AAACGAT37100.05.806017437
CACGACG19550.05.748255715
TACACGA22850.05.59335913
TCCGATC23500.05.42588626
CGACGCT21900.05.030525717
AGTCACG45100.05.02044128