FastQCFastQC Report
Wed 7 Dec 2016
G144-M8-Index-20_GTGGCC_BC9WPLANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG144-M8-Index-20_GTGGCC_BC9WPLANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25869874
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGC19450.07.468786742
GATCTCG20100.07.222320634
GTGGTCG22150.06.35921341
ATCTCGG24900.06.09470335
CGATCGT6202.2460472E-56.03332631
CGGTGGT32100.05.894930439
GTCGCCG26200.05.460535544
TCGGTGG32450.05.152366638
Produced by FastQC (version 0.11.2)