FastQCFastQC Report
Wed 7 Dec 2016
G144-M9-Index-21_GTTTCG_BC9WPLANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG144-M9-Index-21_GTTTCG_BC9WPLANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35978029
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT15350.09.01960744
CGTTTCG16300.08.090878533
CGTACGA7803.274181E-117.34205962
TCGTACG6451.6420017E-87.17201381
ATCGACG6804.7171852E-86.787274441
ACGACGC14350.06.14275616
CGGTTTT41900.06.0985341
TCTCGTA18250.05.90049843
TCGACGC8303.2281423E-75.825208742
CACGACG16900.05.607270215
CGACGCT18200.05.56966317
TCCGATC18600.05.43673226
CGTCGAT6107.2017056E-45.405288724
CGGAATC25950.05.33564538
TCGGAAT26150.05.294896637
TTTCGGA28250.05.135181435
CGGATTA12102.6522684E-85.09746461