FastQCFastQC Report
Fri 24 Mar 2017
G144_M9M10.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG144_M9M10.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79499612
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT35700.09.11097144
CCGTACG23000.08.50564333
CGTACGT26700.07.90323534
ACGACGC36550.07.898028416
CACGACG39600.07.62380815
CGACGCT44450.06.989850517
TACACGA47900.06.671098713
TCTCGTA44200.06.66279143
GTACGTA33200.06.620699435
TCCGATC43750.06.532248526
ATCGACG13850.06.50629841
CTACACG54000.06.162393612
CGGTTTT92950.06.1378761
CGTAATC28150.06.08998738
CGGATTA26150.06.06496861
ACCGTAC37400.05.7598132
CGGTATT54250.05.7251531
CCGATCT50400.05.583160427
CGTACGA15850.05.5584692
ACGCTCT69450.05.515831519
Produced by FastQC (version 0.11.2)