FastQCFastQC Report
Wed 31 Aug 2016
BM-S1-11_S35_L008_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameBM-S1-11_S35_L008_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26003283
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT19300.029.23306744
TCGTATG19800.028.72019845
TCTCGTA18800.027.61926543
GATCGGA22600.025.4446281
ATCGGAA24750.023.4337922
TATCTCG22050.023.34433641
ACACGTC30350.019.25321813
CGTCTGA31250.018.62680816
ATCTCGT28950.018.1684842
ACGTCTG35650.016.70609715
TCGGAAG37650.015.641653
CACGTCT44150.013.64246214
CGGAAGA44150.013.542454
CACACGT45550.013.37117512
GCACACG54300.011.2165211
ACTGATA69300.08.75910134
CTGATAT72750.08.5291735
ATATATC71950.08.09108838
GATATAT71300.08.03997237
ATATCTC70800.07.96851840
Produced by FastQC (version 0.11.2)