FastQCFastQC Report
Wed 31 Aug 2016
BM-S1-13_S37_L008_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameBM-S1-13_S37_L008_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25063376
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA17000.025.52004241
TCGTATG17550.025.36064143
GATCGGA18250.025.0312351
CGTATGC17800.025.0044544
CTCGTAT18150.024.76982342
TATCTCG19800.022.36361539
ATCGGAA21600.021.8552112
ACACGTC24700.018.92592413
CGTCTGA25150.018.76601216
ACGTCTG27200.017.1037815
ATCTCGT26400.016.60246340
TCGGAAG32350.015.0079773
CGGAAGA36000.013.3614614
CACGTCT38100.012.97742214
GCTTATC37900.011.68581536
GTATGCC43850.011.22586445
CACACGT43550.010.99213712
GCACACG48650.010.25559511
TAGCTTA53450.09.12773534
ACTAGCT54600.08.81202232
Produced by FastQC (version 0.11.2)