FastQCFastQC Report
Wed 31 Aug 2016
BM-S1-14_S38_L008_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameBM-S1-14_S38_L008_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24473294
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT11200.016.05595642
TCGTATG11350.015.64587443
CGTATGC11950.014.29599244
ACGGCTA13400.013.08697232
TCTCGTA12050.013.05806141
GATCGGA15050.012.6792951
ATCGGAA15450.011.9309322
ATCTCGT17700.09.65124240
CGGCTAC17750.09.62641633
ACACGTC21050.09.39550713
CGTCTGA20600.08.83705316
ACGTCTG23650.08.17253815
TCACGGC23900.07.806427530
GTCACGG24850.07.507762429
TCGGAAG26850.07.366783
CATCTCG23800.07.272001739
AGTCACG24600.07.126913528
CACGTCT30900.06.473231314
CGGAAGA32450.06.44180544
CACGGCT32800.06.100476331
Produced by FastQC (version 0.11.2)