FastQCFastQC Report
Wed 31 Aug 2016
BM-S1-9_S33_L008_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameBM-S1-9_S33_L008_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34457001
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG6200.029.00977333
CGTACGT6750.026.64558634
ACCGTAC9300.019.58159632
GATCGGA11050.017.9087831
GTACGTA11200.016.46019435
CTCGTAT11050.016.2741444
TCTCGTA12150.014.80076243
TCGTATG12900.014.63628745
CGTCTGA16300.011.99556416
ATCGGAA16200.011.3784322
CACCGTA16450.011.20700431
ATCTCGT17250.010.42474842
CGTAATC18050.09.71309238
GTCACCG20150.09.37066129
AATCTCG19500.09.33726141
ACGTCTG21100.09.26671615
TCACCGT22100.09.25609230
TCGGAAG23200.08.2349983
ACACGTC24250.08.15567713
CGGAAGA25700.07.6963024
Produced by FastQC (version 0.11.2)