FastQCFastQC Report
Fri 5 May 2017
G150-M1-Index-1_ATCACG-_BCALRAANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG150-M1-Index-1_ATCACG-_BCALRAANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21943872
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA13800.018.9811521
CTCGTAT12600.018.6797442
ATCGGAA14500.018.2032972
TCTCGTA12200.018.0299241
TCGTATG13450.017.98876243
CGTATGC14450.016.89623644
ACGATCT14750.014.91533337
ATCACGA17350.014.32919234
GATCTCG16250.013.94281239
ATCTCGT15900.013.83402640
TCACGAT16650.013.74247235
CACGATC16500.013.73371936
CGTCTGA20400.012.61581416
CATCACG20600.012.38883733
ACACGTC20700.012.11556213
CGATCTC18500.011.89057238
ACGTCTG24850.010.35659615
TCGGAAG26900.010.2186763
CACGTCT27850.09.63649214
CGGAAGA29000.09.4032584
Produced by FastQC (version 0.11.2)