FastQCFastQC Report
Fri 5 May 2017
G150-M1_thru_3.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG150-M1_thru_3.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61305288
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT45400.024.86313211
CGTCGTG45800.024.64598312
AGCGTCG48950.022.9700610
TCGTGTA51500.022.04552714
GATCGGA53600.021.138511
ATCGGAA54450.020.6463992
GTCGTGT59400.019.0396813
CGTGTAG62550.018.18639215
GATCTCG58650.015.45270234
GAGCGTC75300.015.370329
AGAGCGT77050.015.1924568
TGGTCGC62000.013.94553842
ATCTCGG66500.013.82706235
TCGGAAG87500.013.2251653
GTGGTCG66350.013.02961141
AAGAGCG92250.012.6414757
CGGAAGA104250.011.1851374
GTCGCCG80550.010.7350244
TCTCGGT88150.010.48098836
TCGGTGG97850.09.95899938
Produced by FastQC (version 0.11.2)