FastQCFastQC Report
Fri 5 May 2017
G150-M1_thru_7.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG150-M1_thru_7.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139099852
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT107300.027.3148611
CGTCGTG108450.027.02521312
AGCGTCG115600.025.31556710
TCGTGTA125800.023.34965314
GATCGGA128700.022.9234371
ATCGGAA132200.022.349342
GTCGTGT139950.020.957613
CGTGTAG150350.019.65433315
GATCTCG141350.017.35213334
GAGCGTC173000.017.1830839
AGAGCGT177850.016.8505158
TGGTCGC141700.015.89875842
ATCTCGG160250.015.51151435
GTGGTCG152250.014.95420241
TCGGAAG202300.014.9094383
AAGAGCG210200.014.4246637
CGGAAGA234850.012.86239054
GTCGCCG184750.012.57643544
TCTCGGT205950.011.97340336
GGTCGCC208950.011.30888943
Produced by FastQC (version 0.11.2)