FastQCFastQC Report
Fri 5 May 2017
G150-M2-Index-2_CGATGT-_BCALRAANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG150-M2-Index-2_CGATGT-_BCALRAANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21387232
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT10400.021.15033342
TCGTATG11350.019.959943
CGATGTA12700.017.32356334
CGTATGC12700.017.14555444
TCTCGTA12500.016.89323241
TATCTCG13050.016.18155939
GATCGGA14950.015.4589521
ACCGATG15300.015.09828532
CACCGAT14900.015.06064731
ATCGGAA16400.014.0824992
CCGATGT16500.014.00048933
ATCTCGT16850.012.92369840
ACACGTC21100.011.260413
CGTCTGA21900.011.24921916
TCACCGA23800.09.79815830
ACGTCTG25300.09.56356115
TCGGAAG28850.08.6132563
GTCACCG31200.07.82648329
CACGTCT32150.07.800129414
CGGAAGA33050.07.31944754
Produced by FastQC (version 0.11.2)