FastQCFastQC Report
Fri 5 May 2017
G150-M4-Index-4_TGACCA-_BCALRAANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG150-M4-Index-4_TGACCA-_BCALRAANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18344229
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT11950.028.53990611
CGTCGTG13350.025.71177712
AGCGTCG13800.025.19216510
GATCGGA15550.022.92011
GTCGTGT15550.022.78128813
TCGTGTA15700.022.56332614
ATCGGAA16400.022.0002272
CGTGTAG19200.018.90891815
AGAGCGT20850.017.2012838
GAGCGTC21200.016.3984979
GATCTCG17250.016.32293534
TGGTCGC17350.015.59678742
GTGGTCG17650.015.20571141
AAGAGCG24550.014.5191637
TCGGAAG26500.013.8640673
ATCTCGG21000.013.8271335
GTCGCCG21550.013.27300944
CGGAAGA29650.012.6143024
TCGGTGG25000.012.14260138
GGTCGCC24400.011.63212443
Produced by FastQC (version 0.11.2)