FastQCFastQC Report
Fri 5 May 2017
G150-M4_thru_7.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG150-M4_thru_7.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77794564
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT67350.027.4012542
TCGTATG68250.027.39279443
CGTATGC71250.026.33210844
TCTCGTA68050.026.18219241
GATCGGA76500.025.434631
ATCGGAA80450.023.7047082
ATCTCGT80300.022.07788540
ACACGTC96550.020.32482313
CGTCTGA97200.019.75640916
ACGTCTG110400.017.55355615
TCGGAAG118450.016.578843
CGCCAAT40200.015.43331733
CACGTCT132750.015.11279914
CGGAAGA134250.014.6773114
TATCTCG45600.013.12066139
CACACGT153600.013.10518612
ACGCCAA55850.011.54179732
GCACACG174600.011.51634711
GTCACGC61300.010.73012429
GATCTCG54900.08.97485339
Produced by FastQC (version 0.11.2)