FastQCFastQC Report
Fri 5 May 2017
G150-M4_thru_7.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG150-M4_thru_7.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77794564
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT64250.028.6303511
CGTCGTG65450.028.10542512
AGCGTCG69900.026.53649910
TCGTGTA75800.024.55733714
GATCGGA77150.024.4100271
ATCGGAA80300.023.506892
GTCGTGT80000.023.24088713
CGTGTAG87700.021.77727715
GATCTCG83050.018.69987934
GAGCGTC100300.018.5372779
AGAGCGT104700.017.9051768
TGGTCGC84350.017.05786742
ATCTCGG94200.016.93015135
GTGGTCG86000.016.72860341
AAGAGCG119800.015.8319717
TCGGAAG123050.015.4117163
GTCGCCG103550.014.36385844
TCTCGGT115150.013.52517936
CGGAAGA141400.013.4901784
TCGGTGG123250.013.36798138
Produced by FastQC (version 0.11.2)