FastQCFastQC Report
Fri 5 May 2017
G150-M5-Index-5_ACAGTG-_BCALRAANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG150-M5-Index-5_ACAGTG-_BCALRAANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20417999
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT16800.026.84898411
CGTCGTG16800.026.58704212
AGCGTCG18450.023.97076410
TCGTGTA19150.022.63456714
ATCGGAA20850.022.1583272
GATCGGA21100.021.7916811
GTCGTGT21100.020.64717913
CGTGTAG22200.020.41701115
GATCTCG20700.017.428234
TGGTCGC19300.016.64297542
GAGCGTC27000.016.6243389
AGAGCGT27300.016.6028068
ATCTCGG23050.016.1285335
TCGGAAG31850.015.1273423
GTGGTCG23450.014.54054441
AAGAGCG32000.014.2329917
CGGAAGA36150.013.7547794
TCTCGGT28500.012.5811936
GTCGCCG28450.012.06459844
TCGGTGG32950.011.28262838
Produced by FastQC (version 0.11.2)