FastQCFastQC Report
Fri 5 May 2017
G150-M6-Index-6_GCCAAT-_BCALRAANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG150-M6-Index-6_GCCAAT-_BCALRAANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19158965
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT20800.031.72532542
TCGTATG21600.030.34474243
CGCCAAT21900.030.03788433
TCTCGTA21250.029.8115941
CGTATGC22600.029.29478844
GATCGGA23950.028.5798841
TATCTCG22850.028.4947639
ATCGGAA24650.028.018292
ACGCCAA25900.025.48304432
ATCTCGT26000.024.618440
GTCACGC28050.023.6846829
CGTCTGA28950.023.63029116
ACACGTC30050.022.18188713
ACGTCTG31250.021.89104515
AGTCACG32400.021.04769528
TCACGCC32500.020.64584530
TCGGAAG38000.018.402183
CACGCCA37650.018.23137531
CACGTCT37300.018.16428814
CGGAAGA39650.017.1380964
Produced by FastQC (version 0.11.2)