FastQCFastQC Report
Fri 5 May 2017
G150-M7-Index-7_CAGATC-_BCALRAANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG150-M7-Index-7_CAGATC-_BCALRAANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19873371
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT14900.026.12954742
TCTCGTA14500.025.63672341
TCGTATG15350.025.5048443
GATCGGA17000.023.9529231
CGTATGC17450.022.56181744
ATCGGAA18200.022.478982
ATCTCGT18600.020.22238540
ACACGTC22050.018.45766813
CGTCTGA22350.018.10925116
ACGTCTG24350.016.62179615
CATCTCG23850.015.95609739
TCGGAAG27500.014.633513
CGGAAGA30150.013.49362854
CACGTCT31900.012.96430414
CACACGT36500.011.57236412
GATCATC37400.010.52969836
GCACACG40200.010.50724611
ACCAGAT58450.07.452688732
AGATCAT57800.07.041891635
GAACTCC64950.06.980904621
Produced by FastQC (version 0.11.2)