FastQCFastQC Report
Fri 5 May 2017
G150-M7-Index-7_CAGATC-_BCALRAANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG150-M7-Index-7_CAGATC-_BCALRAANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19873371
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT13800.028.22189711
AGCGTCG14200.027.11700610
CGTCGTG14900.025.99072612
GATCGGA16850.023.3710631
ATCGGAA17200.023.1510732
TCGTGTA17000.023.03839314
GTCGTGT18600.021.17513
CGTGTAG19900.019.68109115
GATCTCG18100.017.8653134
AGAGCGT22400.017.3866188
GAGCGTC22350.017.2287039
ATCTCGG20650.016.51138335
AAGAGCG27200.015.450887
GTGGTCG17850.015.28162241
TGGTCGC17450.015.25549242
TCGGAAG27850.014.3769993
CGGAAGA31500.012.7817074
GTCGCCG20900.012.52790644
TCTCGGT27700.011.83254936
TCGGTGG29100.011.56562638
Produced by FastQC (version 0.11.2)