FastQCFastQC Report
Fri 5 May 2017
G150-M15-Index-15_ATGTCA-_BCALRAANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG150-M15-Index-15_ATGTCA-_BCALRAANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24578938
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG9150.031.985312
GCGTCGT9900.029.11768511
AGCGTCG10500.028.08253310
GTCGCCG10950.023.51019744
GATCGGA13600.021.3535061
TCGTGTA16750.017.99787514
TGGTCGC13250.017.76538742
ATCGGAA16700.017.6532332
GAGCGTC17150.017.5779559
GTCGTGT16900.017.57779113
GATCTCG16150.016.34438934
GGTCGCC17100.015.05410143
CGTGTAG20250.014.99573515
AGAGCGT20900.014.634298
GTGGTCG17550.013.66195541
ATCTCGG20850.013.18738235
AAGAGCG23650.012.8396397
TCGGTGG22100.012.640438
TCGGAAG23900.012.6124953
TCTCGGT23200.010.99823236
Produced by FastQC (version 0.11.2)