FastQCFastQC Report
Fri 5 May 2017
G150-M15_thru_17.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG150-M15_thru_17.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69762233
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA40100.018.355231
ACGTCCG16450.017.89978432
CTCGTAT36150.016.77433244
ATCGGAA45700.016.768842
CGTCCGC17950.016.52638633
TCTCGTA36400.015.45062243
CGTCTGA57950.013.45137716
ACACGTC59750.012.82479513
ACGTCTG68250.011.22698715
ATCTCGT52300.011.08880542
TCGGAAG71600.010.9796383
GTCCGCA31150.010.36979234
CGGAAGA82000.09.6951714
CACGTCT91200.08.78818314
TCCGCAC37150.08.69465235
AATCTCG34900.08.56051241
CACACGT113350.07.129390712
GCACACG119150.07.00370911
CGCACAT46450.06.57456537
CCGCACA50350.06.502319336
Produced by FastQC (version 0.11.2)