FastQCFastQC Report
Fri 5 May 2017
G150-M15_thru_17.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG150-M15_thru_17.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69762233
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG27100.026.7144412
AGCGTCG28250.025.70491410
GCGTCGT29000.024.81249611
GTCGCCG29950.019.83596844
GATCGGA39050.018.5356731
ATCGGAA45750.016.0616722
GTCGTGT46300.015.77879113
TCGTGTA46450.015.77516614
GAGCGTC49350.015.160189
TGGTCGC40150.014.41034742
GATCTCG46850.013.61589834
CGTGTAG54700.013.55679515
GTGGTCG46300.012.68510441
AGAGCGT61550.012.2980298
GGTCGCC51000.011.95006343
ATCTCGG57250.011.71861635
AAGAGCG69500.010.9546137
TCGGAAG70600.010.7520943
TCGGTGG68550.09.97928938
TCTCGGT65150.09.89244536
Produced by FastQC (version 0.11.2)