FastQCFastQC Report
Fri 5 May 2017
G150-M15_thru_21.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG150-M15_thru_21.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162404554
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG64500.026.88312312
GCGTCGT65950.026.12533211
AGCGTCG66650.026.04903610
GTCGCCG69850.020.19181844
GATCGGA89850.019.295041
TCGTGTA106550.016.58331314
GTCGTGT109000.016.4125113
ATCGGAA106350.016.384282
GAGCGTC113400.015.7755529
TGGTCGC92550.015.07021542
CGTGTAG124000.014.39152815
GATCTCG108250.014.14299134
GTGGTCG108550.013.11114741
AGAGCGT143050.012.6286268
GGTCGCC117800.012.43916443
ATCTCGG135750.011.650475535
AAGAGCG161450.011.2984197
TCGGAAG169850.010.66130353
TCGGTGG160000.010.22826438
TCTCGGT153650.010.1070736
Produced by FastQC (version 0.11.2)