FastQCFastQC Report
Fri 5 May 2017
G150-M16-Index-16_CCGTCC-_BCALRAANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG150-M16-Index-16_CCGTCC-_BCALRAANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23557228
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA10200.016.8521421
TCGTATG10800.016.0700743
TCTCGTA10600.014.92175341
ATCGGAA12050.014.6192562
CTCGTAT11950.014.15502142
CGTATGC12200.014.04687144
ACCCGTC14700.011.80946432
ACACGTC19100.010.49101313
CGTCTGA18350.09.95969116
CGTCCAT16750.09.83896435
CCGTCCA18500.09.74010234
ATCTCGT16250.09.59844740
CACCCGT19200.09.27052331
TCACCCG18550.09.12105730
TCGGAAG21400.09.0548653
ACGTCTG21700.08.42166915
CATCTCG19400.08.37966939
CGGAAGA25200.07.8645944
CCCGTCC27400.07.458523333
CACGTCT27900.06.78708514
Produced by FastQC (version 0.11.2)